2006 Supplementary Materials for Manuscripts

  • Pohl et al 2006. Am. J. Vet. Res. 67: 616-626
    Pohl, M. A., K. K. Nightingale, M. Wiedmann. 2006. Listeria monocytogenes genotypes are associated with distinct clinical manifestations of listeriosis in cattle. American Journal of Veterinary Research 67(4): 616-626.
  • Tsai et al. 2006. Infection, Genetics and Evolution 6:378-389.
    Tsai, Y-H. L., R. H. Orsi, K. K. Nightingale and M. Wiedmann. 2005. Listeria monocytogenes internalins are highly diverse and evolved by intragenic recombination and positive selection. Infection, Genetics and Evolution (e-pub)
  • Garner et al., 2006. Appl. Environ. Micro. 72:5384-5395
    M. R. Garner, K. E. James, M.C. Callahan, M. Wiedmann, and K. J. Boor. 2006. Exposure to salt and organic acids increases the ability of Listeria monocytogenes to invade Caco-2 cells but decreases L. monocytogenes’ ability to survive gastric stress Appl. Env. Micro. Submitted
    • Supplementary Table ST1 Doubling times of L. monocytogenes cultured in the presence of food additives.
    • Supplemental Fig.S1. Evaluation of L. monocytogenes growth in the presence of salt and organic acids using OD600 measurements.
    • Supplemental Fig.S2. Evaluation of L. monocytogenes growth in the presence of salt and organic acids using OD600 measurements.
    • Supplemental Fig.S3 Viability of L. monocytogenes 10403Sāˆ†sigB inoculated into BHI broth containing food additives.
    • Supplemental Fig.S4 Evaluation of L. monocytogenes growth in the presence of salt and organic acids using OD600 measurements using a low starting concentration (~5x101 CFU/ml) of L. monocytogenes
    • Supplemental Fig. S5 Box plot of L. monocytogenes 10403S invasion efficiency into Caco-2 tissue culture cells inoculated with varying L. monocytogenes concentrations.
    • Supplemental Fig. S6. Box plot of L. monocytogenes invasion efficiency into Caco-2 tissue culture cells for (i) L. monocytogenes strains grown to stationary phase in the presence of no additives, NaCl, or SL, and (ii) L. monocytogenes grown to stationary phase in BHI alone but added to Caco-2 cells with filter sterilized supernatant from L. monocytogenes grown in the presence of no additives, NaCl or SL (thus adding SL or NaCl in BHI as well as bacterial supernatant molecules).
    • Supplemental Fig. S7 Box plot of L. monocytogenes invasion efficiency into Caco-2 tissue culture cells with (i) L. monocytogenes grown to stationary phase in the presence of no additives, NaCl, or SL, and (ii) L. monocytogenes strains grown to stationary phase in the presence of no additives, NaCl, or SL with infections performed by adding L. monocytogenes cells as well 100µl synthetic gastric fluid pH 4.5 to Caco-2 cells.
  • Alcaine et al., 2006 Appl. Environ. Micro. 72:7575-7585
    S. D. Alcaine, Y. Soyer, L. D. Warnick, W.-L. Su, S. S. Sukhnanand, J. Richards, E. Fortes, P. McDonough, T. Root, N. B. Dumas, Y. Groehn, and M. Wiedmann. 2006. Multilocus sequence typing supports that bovine and human associated Salmonella represent distinct and overlapping populations, including clonal groups with duplication and deletion events in genes encoding cell surface molecule associated proteins. J. Clin. Micro. (in preparation)
  • Ho et al., 2006. J. Dairy Sci. 90:2517-2524
    A. J. Ho, V. R. Lappi, and M. Wiedmann. 2006. Longitudinal monitoring of Listeria monocytogenes contamination patterns in a farmstead dairy processing facility. J. Dairy Sci. 90(5): 2517-2524.
  • Huck et al. 2006. J. Food Prot. 70:2354-64
    J.R. Huck, N.H. Woodcock, R.D. Ralyea, and K.J. Boor.  2006. Molecular subtyping and characterization of psychrotolerant endospore-forming bacteria in two New York State fluid milk processing systems.  J. Food Prot. (submitted)
    • Supplemental Table 1. 16S rDNA, rpoB and combined sequence type data for isolates subtyped in this study.
    • Supplemental Table 2.  Contamination patterns by endospore-forming bacteria in NYS dairy plant U over three consecutive weeks of sampling.
    • Supplemental Table 3.  Contamination patterns by endospore-forming bacteria in NYS dairy plant M over three consecutive weeks of sampling.